Protein Analysis Reagents
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
Thermo Scientific Chemicals RIPA buffer (5X)
RIPA buffer, radio immunoprecipitation assay buffer, is used for whole cell isolation of proteins from tissues and cell culture.
Invitrogen™ Magnetic Stand-96
The stand has clear polyacryl base in standard SBS 96-well microplate footprint and uses 24 extremely strong permanent magnets for fast, easy magnetic separation in little as 30 seconds.
Invitrogen™ Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Thermo Scientific™ Pierce™ Protein G Magnetic Beads
Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.
Thermo Scientific™ Pierce™ 2-Mercaptoethanol
Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).
Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent
Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.
Gibco™ PNGase F Glycan Cleavage Kit
PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.
Invitrogen™ Ionomycin, Calcium Salt
Ionomycin is an effective Ca2+ ionopohre that is commonly used to both modify intracellular Ca2+ concentrations and to calibrate fluorescent Ca2+ indicators when studying the regulatory properties of Ca2+ in cellular processes.
Invitrogen™ Streptavidin, R-Phycoerythrin Conjugate (SAPE) - 1 mg/mL
Streptavidin, R-Phycoerythrin Conjugate (SAPE) - 1 mg/mL
Thermo Scientific™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent
Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent.
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Invitrogen™ Streptavidin, Alexa Fluor™ 647 Conjugate
Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye).